For this project, we were approached by the client to create an in-depth technical manual to document their complex protocol for the large-scale production of phytocannabinoids from cell culture. The manual details the clients’ proprietary and patent-protected process for subculturing from donor plant tissue, scaling up to high-volume cell culture in 1000-L vats, media filtration, and phytocannabinoid recovery. Our work on the project involved months of collaboration with the lead scientists, several trips to the laboratory, and familiarization with each step of the protocol, every fitting, and all laboratory equipment involved. The collaboration resulted in an 86-page Phytocannabinoid Production Manual that included an overview of the process, best practices for cell culture and sterilizing lab equipment, step-by-step instructions that could easily be repeated by a skilled laboratory technician, figures and associated captions to guide the user, and appendices with recipes for all necessary solutions. This process targets the desired qualities (i.e., phytocannabinoid composition) of each donor plant using only a few mm2 of live flower tissue and mitigates prevailing issues with conventional agricultural or hydroponic phytocannabinoid production, which generally require large-scale grow operations to produce similar quantities of phytocannabinoids. Thus, this innovative technology has the potential to revolutionize the production of both phytocannabinoids and terpenes for large-scale medicinal use.
Technical Writing and Illustration Development
Bahku Holdings/Cell Science Ltd.